At Synbio Technologies we pride ourselves in being one of the premier companies within not only the gene synthesis industry, but the biotechnology industry as a whole. This confidence is relying upon our tested and proven methods of gene synthesis. Gene synthesis can be defined as the method in synthetic biology used to engineer an […]
Tag: codon optimization
Gene Synthesis Definition
The traditional method of studying a gene’s function is to extract the gene of interest, amplify it and put the resulting sequence through various molecular assessments. These assessments can vary from generating a frameshift mutation within the sequence, to upregulating the expression of the sequence in order to observe the resulting phenotypes the mutated sequence […]
Large Gene Synthesis
An interesting and important aspect of genetic research that has grown drastically in popularity over the last ten to fifteen years is gene synthesis . Gene synthesis can be defined as the construction of a physical genetic sequence from a requested sequence in text format. Gene synthesis allows us, at Synbio Technologies, to manufacture genetic […]
Gene Synthesis Vectors
A relatively recent discovery, dating back to the early 1970s, in biology has revolutionized the field of genetics research. This discovery is known as gene synthesis , which allows for the manufacturing of a physical genetic sequence from a string of base pairs in text format. Gene synthesis allows for the creation of a genetic […]
Gene Synthesis Mutagenesis
One of the fundamental ways to study the function of a gene is to void, knockout, its function and observe the resulting phenotype. This void of function, commonly known as mutagenesis, has been practice within the field of genetics for many years and has been proven effective countless times. A more commonly utilized method of […]
Gene Synthesis Cost
Moore’s Law has been applied and shown in various industries ranging from the tech industry to the biotech industry. Moore’s Law has most notably shown within the field of genetics when analyzing the cost of sequencing the human genome. The first whole genome sequencing was conducted on a human in the early 2000s, and costed […]
Gene Synthesis by PCR
Prior to the 1970s traditional cloning was used in order to amplify a gene of interest. This process of gene synthesis by PCR is still used today, but relies on a laborious process of achieving the final amplified product. Traditional cloning relies on recombinant DNA being integrated into a bacteria host’s genome. Once the DNA […]
Gene Synthesis Codon Optimization
Gene synthesis refers to the technology of artificially synthesizing double-stranded DNA molecules in vitro through reverse transcription of mRNA of a known template gene. Intelligent synthetic gene design is critical to gene engineering and the efficient production of recombinant protein through heterologous hosts. Unfortunately, not all genes can be successfully and effectively expressed in heterologous […]
Synbio Technologies’ Gene Synthesis Process
Synbio Technologies’s proprietary Syno®2.0 gene synthesis process includes computational design of short oligos, which can then be reliably synthesized, assembled, and cloned into the desired vector. An available precursor to this process is optimization of the gene sequence for improved protein expression and other purposes. Fig. 1 Syno®2.0 gene synthesis processA large number of oligos […]
Codon Usage Bias
The genetic information in DNA is transcribed into mRNA then translated into protein, in which codons played important roles during the process. Total 64 combinations of nucleotide triplet (codon) encode all 22 amino acids. Each amino acid corresponds to at least one codon, e.g. methionine and tryptophan. In other cases, amino acids are encoded by […]
Synbio Technologies’ Codon Optimization Strategy
Translation is the last step in protein synthesis that results in stable proteins from DNA replication, mRNA transcription and modification in cells. Codon optimization aims to increase the yield of protein expression in different organisms by rebalancing usage of synonymous codons. High quality protein has a wide range of applications, including study of protein structure […]
Synbio Technologies’ NGTMCodon Optimization Software
Synbio Technologies’ proprietary NGTMCodon optimization software provides customers with free codon optimization services bundled with gene synthesis. Our NGTM Codon optimization software optimizes gene sequences based on different expression systems to maximize protein expression. NGTMCodon optimization software advantages： Elimination of codon usage bias: Codon usage bias refers to the differences in frequency of synonymous codons […]
