CRISPR-Cas9 sgRNA Library
CRISPR-Cas9/gRNA (CRISPR-Cas RNA-Guided nuclease) is considered as the biggest breakthrough in genome editing technology after TALEN and ZFN. It precisely edits DNA through the RNA-directed Cas9 nuclease. Previous studies indicated that the on-target gene knockout rates were higher than that of TALEN and ZFN. Besides, the construction of sgRNA-Cas9 is easier and more convenient. It has been widely applied to animal and plant breeding, directed evolution, and the site-specific restoration of genetic diseases. Synbio Technologies offers the designing, construction and validation of CRISPR-Cas9 gene targeting system through our patent pending synthetic biology platform in a highly cost and time-effective approach. We offer one station solutions for CRISPR Cas9 projects, including genome scale gene targeting.
Advantages of CRISPR-Cas9
- Appliocable in any type of cell lines
- Simultaneously knockout multiple targets
- More efficient
- Fast turnaround time
Applications
- CRISPR-Cas9-based gene knock-out
- CRISPR-Cas9-based gene knock-in
- CRISPR-Cas9-based transcription activation
- CRISPR-Cas9-based transcription inhibition
Services and turnaround time
- CRISPR-Cas9-based primer design
- CRISPR-Cas9-based plasmid construction
- CRISPR-Cas9-based target locus validation
- CRISPR-Cas9-based stable cell line construction
- CRISPR-Cas9-based stem cell construction
| Service type | Price | Turnaround time(business days) |
| CRISPR-Cas9-based primer design | Quote | 1-3 |
| CRISPR-Cas9-based plasmid construction | Quote | 10 |
| CRISPR-Cas9-based target locus validation | Quote | Quote |
| CRISPR-Cas9-based stable cell line construction | Quote | Quote |
| CRISPR-Cas9-based stem cell construction | Quote | Quote |
Delivery Form
- Certificate of analysis (COA) for project
- Lyophilized plasmid DNA (4µg), or libraries
- Sequencing chromatogram
- QC report
How to order
 Tel +1 732-230-3003 |
 service@synbio-tech.com |
 Fax +1 609 228 5911 |
 Online Inquiry online inquiry submission form |