Polymerase chain reaction, generally known as PCR and also referred to as in vitro DNA amplification, is one of the most widely used techniques in biology labs across the world. Traditional DNA amplification method Traditional DNA amplification involves the construction of a vector containing the target gene, followed by the transfer of the vectors into […]
Tag: Synbio Technologies Wiki
Codon Usage Bias
The genetic information in DNA is transcribed into mRNA then translated into protein, in which codons played important roles during the process. Total 64 combinations of nucleotide triplet (codon) encode all 22 amino acids. Each amino acid corresponds to at least one codon, e.g. methionine and tryptophan. In other cases, amino acids are encoded by […]
Synbio Technologies’ Codon Optimization Strategy
Translation is the last step in protein synthesis that results in stable proteins from DNA replication, mRNA transcription and modification in cells. Codon optimization aims to increase the yield of protein expression in different organisms by rebalancing usage of synonymous codons. High quality protein has a wide range of applications, including study of protein structure […]
Synbio Technologies’ NGTMCodon Optimization Software
Synbio Technologies’ proprietary NGTMCodon optimization software provides customers with free codon optimization services bundled with gene synthesis. Our NGTM Codon optimization software optimizes gene sequences based on different expression systems to maximize protein expression. NGTMCodon optimization software advantages： Elimination of codon usage bias: Codon usage bias refers to the differences in frequency of synonymous codons […]
Four Steps of Subcloning Technology Protocol
Subcloning refers to the technique of re-cloning a DNA fragment from one vector to another, so that we can more easily perform analysis, transformation, and recombination of the target gene(s). Subcloning is an important tool in any molecular biologist’s toolkit, helping to elucidate the function of a target gene and to easily analyze its phenotype. […]
Synbio Technologies’ PCR cloning and Subcloning Technology
PCR cloning and subcloning technology, first developed in the 1970s, is now a staple in every molecular biology lab in the world. Cloning allows researchers to much more easily understand gene function at a deeper level, and greatly facilitates gene editing. PCR cloning and subcloning technology is not only revolutionary for the field of biology, […]
Genome Editing Technology vs Transgenic Technology
Genome editing technology Genome editing technology enables genetic engineering where DNA is replaced, deleted or inserted in the genome of a living organism, and the emergence of CRISPR-Cas9 system has further facilitated the realization of precise genetic modifications. Gene mapping and precise genetic modifications by inducing targeted DNA double-strand breaks opened up new avenues for […]
The Comparison Between Three Generation Genome Editing Technologies
Genome editing refers to a type of genetic engineering in which DNA is replaced, deleted or inserted in the genome of a living organism using engineered nucleases. These engineered nucleases enable efficient and precise genetic modifications by inducing targeted DNA double-strand breaks (DSBs) that stimulate the cellular DNA repair mechanisms, There are currently three families […]
The Applications of Genome Editing Technology
Genome editing technology enables manipulations at genome level where DNA is replaced, deleted or inserted in a living organism. Classic genome editing approaches depend on homology-directed repair and the totipotency of stem cells to facilitate the modification of the individual gene. The Nobel Prize in Physiology or Medicine 2007 was awarded to the discoveries of […]
A Brief Introduction of Molecular Biology Techniques
Molecular biology studies the morphology, structural features, biological relevance and regulatory mechanism of biological macromolecules including nucleic acid, protein and others. Molecular biology truly uncovered the mysteries of the biological world from the molecular level; it signaled the transition from passive adaption from nature to actively restructuring and reengineering the nature. The main research contents […]
The Wide Applications of Molecular Biology
Molecular biology clarifies regularities of cell and receals the essence of life.Molecular biology technologies have been widely adapted for recombinant protein production, genetic modification of organisms, gene therapy, environmental protection, etc. Production of recombinant proteins Recombinant Insulin production For very long time, the insulin that was used to treat diabetic patients is solely purified from […]
Applications of Molecular Biology Techniques in Environmental Microbiology Research
Advances in molecular biology and genetic engineering technology, microbial genetic manipulations have promoted the application of microorganisms in in ecological and environmental research. Genetically engineered microorganisms are being developed and assessed for their beneficial uses in environmental monitoring, toxic chemicals pollution control and genetically engineered microorganisms. Hybridization Probe The oligonucleotide probes can hybridize to DNA […]
The Multiple Application of CRISPR-Cas9
CRISPR-Cas9 gene editing technology can target virtually any genomic location of choice by utilizing a short RNA guide, then cut the sequence with the Cas9 protein. Compared with traditional gene editing technologies, the CRISPR-Cas9 system is both faster and more efficient. The CRISPR-Cas9 system currently has a wide variety of applications in gene engineering and […]
The History of CRISPR-Cas9
The CRISPR (Clustered regularly interspaced short palindromic repeats) system was first identified in archaea as an adaptive defensive mechanism that confers resistance to foreign genetic elements. Later on, the CRISPR-Cas system was engineered into a versatile gene-editing tool enabling manipulation of protospacer adjacent motif (PAM) downstream DNA. Now, CRISPR-Cas9 has facilitated robust genome editing in […]
What Are the Advantages of CRISPR-Cas9?
The CRISPR-Cas9 system is perhaps the most remarkable recent breakthrough in genome editing technology. CRISPR is a ubiquitous family of clustered repetitive DNA elements present in 90% of Archaea and 40% of sequenced Bacteria. CRISPR arrays consist of interspersed identical REPEAT sequences (21-48bp) and several unique invader-targeting SPACER sequences (26-72bp). The CRISPR-Cas9 genome editing system […]
Synbio Technologies’ DNA Synthesis Service
DNA synthesis is a method in synthetic biology for the creation of deoxyribonucleic acid biopolymers in a sequence-specific manner. DNA synthesis can be further classified into oligonucleotide synthesis and gene synthesis. Currently, oligonucleotides are manufactured almost exclusively using automated solid-phase methods.In this way, we can apply efficient and accurate DNA synthesis services. Oligonucleotide synthesis produces […]
Next Generation DNA Synthesis Company
As a leading DNA synthesis company, Synbio Technologies offers a comprehensive package of synthetic genomics services, from simple oligonucleotides and DNA fragments to large and complex genome clusters. Our cutting edge Syno® 3.0 high-throughput platform is the world’s most advanced next-generation DNA synthesis platform, able to provide affordable synthetic DNA at unprecedented scale. At Synbio […]
DNA Synthesis Application
With the growth of DNA synthesis techniques, especially the focus on automation and automatic technologies, we can more easily, rapidly, and efficiently synthesize DNA fragments. The applications of synthetic DNA encompass a broad spectrum ranging from genetic circuits and metabolic pathways to synthetic genomes. DNA synthesis is an essential technique in molecular biology and plays […]
Synthetic Biology Services
Synbio Technologies is devoted to synthetic biology and the development of related products. Synthetic biology facilitates tasks like editing an existing gene/genome or redesigning a novel metabolism pathway, to suit any and all consumer needs. Our synthetic biology services cover a broad range of DNA technologies, including reading, writing, and editing of DNA. Synthetic biology […]
Synthetic Biology Definition
Synthetic biology refers to the design and construction of novel biological components, systems, and technologies through logic and existing biological knowledge. Synthetic biology is a relatively new discipline, emerging during the 20th century. Since then, many breakthroughs have been achieved in research on the synthesis of biological materials, especially in recent years. Compared to traditional […]
