Genome Editing in Mammalian Cells
CRISPR-Cas9 genome editing has an extremely large impact on research field of life science. The CRISPR-Cas9 genome editing system was first successfully utilized in the late 1980s, and since then it has risen in not only popularity, but also in effectiveness. When compared to traditional methods of genome editing, zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN), CRISPR-Cas9 exceeds both of their outdated and inefficient mechanisms. CRISPR-Cas9 was shown in recent research to successfully produce a much higher on-target gene knock-out rate, as we as an economical advantage over ZFN and TALEN. For these reasons, the method most commonly chosen to successfully conduct genome editing within the research fields of life sciences, especially mammalian research, is through use of the CRISPR-Cas9 system. One pivotal topic within biomedical research that CRISPR-Cas9 has improved drastically is successfully generating specific genome modifications within mammalian cells. The resulting genome editing in mammalian cells can then be used as animal models which are immensely useful in modern biomedical research. CRISPR-Cas9 is the most effective way to understand human diseases without the cost and inconvenience of using human cells. The biomimetic properties of animal cells make them extremely useful for studying the mechanism, occurrence, and development of many human diseases. With this combined data we hope to further our growing knowledge of human disease and mechanisms to counteract their effects.
Synbio Technologies can provide genome editing in mammalian cells, including CRISPR-Cas9 sgRNA design, synthesis, activity detection, package into lentivirus, transfer into cells and specific gene knock in/out. With this combination of various services, we are confident in our ability to provide our customers with a specific approach to accomplish their research goals.
Mammalian Genome Editing Service Process
Advantages of Mammalian Genome Editing
- Wide range of applications:
- Simple and convenient to construct:
- Efficient turnaround time:
- Multiple site knock out:
CRISPR-Cas9 genome editing technology has no restrictions among genomic sequences, cell types, or species.
CRISPR-Cas9 requires only a short sequence, approximately 20bps in length, of the sgRNA sequence to complete target recognition.
Genetic stability of homozygous strains can be constructed within an efficient timeframe (Time Frame).
Multiple sites can be targeted at the same time to successfully accomplish multi-site knockout.
Mammalian Gene Editing Service Program:
- sgRNA Endogenous Activity Detection
- Lentiviral Package
- Small Animal Gene Knock-out
1、A variety of sgRNA endogenous activity assays guarantee the efficiency of the generated gene knock-out.
2、The methods of detection include: SSA activity detection, in vitro cleavage activity detection, and endogenous activity detection.
Lentiviral package services offer: transfection of mammalian cells, facilitation of stable Cas9 protein-expressing cell lines, and improving gene knock-out efficiency.
With our experienced team of R&D engineers, Synbio Technologies is capable of providing gene knockout services for zebrafish, mouse, and rat genomes.
CRISPR-Cas9 Genome Editing Frequently Asked Questions
- What Are the Advantages of CRISPR-Cas9 genome editing? Read more
- How was the CRISPR-Cas9 system found? Read more
- How to apply the CRISPR-Cas9 system？Read more
CRISPR-Cas9 Genome Editing Related services
- sgRNA design and synthesis：
- CRISPR Cas9 sgRNA Library Design and Screening：
- CRISPR-Cas9 sgRNA Panel design and construct targeting a metabolic pathway or a series of related genes.
- Yeast Genome Editing platform provides a one-step service for highly accurate editing.
Well-designed sgRNA sequences with lowest off-target rate
fast and efficiently construct sgRNA library with proprietary Syno® 3.0 high-throughput platform
How to order Mammalian Genome Editing
If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.
 Online Inquiry |
 service@synbio-tech.com |
 +1 732-230-3003 |
 +1 609 228 5911 |
References:
[1].Shen, B., et al., Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nature Methods, 2014. 11(4): p. 399-402.
[2].Zou, Q., et al., Generation of gene-target dogs using CRISPR/Cas9 system. Journal of Molecular Cell Biology, 2015. 7(6).
[3].Liang, P., et al., CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein & Cell, 2015. 6(5): p. 363-372.